Abstract:

Capillary electrophoresis(CE)has become a popular separate and analytical technique through the development for 20 years since it was introduced in the early of 1980s.With the combination of traditional elec-trophoresis and modern high performance liquid chromatography(HPLC), CE has been widely used in life science,biochemistry, environmental science, medical science and pharmaceutical chemistry with the advantageous aspects of higher separation effciency, smaller sample volume, lower operation cost and stronger resistance to interferences.The separation of enantiomers,which demands high sensitivity, highselectivity and high separation efficiency, is main challenge to chemistry, especially analytical chemistry.Chromatography techniques, including HPLC, thin layer chromatography(TLC), gas chromatography(GC)and high performance capillary electrophoresis(HPCE), are com-monly used for the chiral separation.However, the disadvantages of long analytical time, expensive chiral column,complicated manipulation and the large volume of solvent waste have limited the application o HPLC,which is the most universal chiral separation method.The rapid developing CE technique has become an attractive alternative for the chiral separation and has been applied to many resarch fields.Amino acids are the fundamental units of protein which is the main component of the living organisms.The sep-aration and analysis of biological moleculars, including amino acid, polypeptide, DNA, nucleic acid and protein, are attractive and difficult topics in analytical chemistry and life science.Therefore, it has momentous academic and clin-ical practice significance to develop simple, fsat, accurate, sensitive methods for esparation and analysis of aminoacids and investigate the relationships between amino acids and senescence, diabetes, liver cancer and coronary dis-eases.The major contents of this dissertation are described in the following chapter.

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