Expression analysis of PaMT from Phytolacca Americana

doi:10.7523/j.issn.2095-6134.2012.4.003

Abstract

Abstract: A full-length cDNA of metallothionein, designated PaMT, was cloned from SSH libraries. PaMT consists of 141 bp that code 46 amino acids. Classic structure (cxc) of metallothionein and the phylogenetic tree analysis show that PaMT belongs to metallothionein family. PaMT is preferentially expressed in root and its expression is strongly up-regulated by heavy metal stress. PaMT-NusA fusion protein expressed in Coli BL21 plays a role in the tolerance to heavy metal toxicity.

Key words: Phytolacca Americana, heavy metal, metallothionein

CLC Number:

Q943.2

WANG Xiao, ZHAO Hui-Jun, LIU Hui-Min, CHAI Tuan-Yao. Expression analysis of PaMT from Phytolacca Americana[J]. , 2012, 29(4): 449-454.

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