Confirmation of weak protein-protein interactions by optimizing co-immunoprecipitation

doi:10.7523/j.issn.1002-1175.2013.01.004

Abstract

Abstract:

Co-immunoprecipitation is widely used to detect protein-protein interaction in physiological condition. The single-factor method was used to optimize some factors of co-immunoprecipitation, including component concentrations of cell-lysis buffer, antibody dosage, hCLP46 protein quantity, and cross-linking DSP. As a result, the weak interaction between hCLP46 (human CAP10-like protein 46) and ER chaperone calnexin is confirmed. The present work provides an important basis for further study of the protein-protein interaction.

Key words: co-immunoprecipitation(Co-IP), hCLP46, calnexin, optimization factors, protein-protein interaction

CLC Number:

FENG Xiao-Qin, XU Feng, WANG Wei, LIU Li-Xin. Confirmation of weak protein-protein interactions by optimizing co-immunoprecipitation[J]. , 2013, 30(1): 18-23.

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