Expression of PaEXP1 from Phytolacca acinosa seedling in response to adversity

doi:10.7523/j.issn.2095-6134.2010.4.003

Abstract

Abstract:

With a full length cDNA of expansin, designed PaEXP1, was cloned in Phytolacca acinosa by the Homology-based cloning method. The PaEXP1 consisted of 1128 bp with an open reading frame of 759 bp that code a protein of 252 amino acids. Structural analysis showed that the eight cysteine residues and the HFD(His-Phe-Asp) sequence are near the N-terminal end, four tryptophan residues near the C-terminal end of predicted PaEXP1. The phylogenetic tree showed that PaEXP1 belonged to the α-expansion protein family. PaEXP1 was preferentially expressed in root. The expression of PaEXP1were strongly inhibited by various abiotic stress, such as the low temperature, salt, heavy metals and osmotic stress. The expression of PaEXP1 in yeast increased the average diameter of yeast, indicating PaEXP1 involved in cell wall expansion.

Key words: Phytolacca acinosa, PaEXP1, expression

CLC Number:

Q943.2

WANG Hui, SUN Tao, ZHAO Hui-Jun, CHAI Tuan-Yao. Expression of PaEXP1 from Phytolacca acinosa seedling in response to adversity[J]. , 2010, 27(4): 448-455.

References

[1] Xue S G, Chen Y X, Lin Q, et al. Phytolacca acinosa Roxb. (Phytolaccaceae): A new manganese hyperaccumulator plant from Southern China
[J]. Acta Ecologica Sinica, 2003, 23(5): 935-937(in Chinese). 薛生国, 陈英旭, 林琦, 等. 中国首次发现的锰超积累植物——商陆
[J]. 生态学报, 2003, 23(5): 935-937.

[2] Nie F. Cd hyper-accumulator Phytolacca acinosa Roxb and Cd-accumulative characteristics
[J]. Ecology and Environment, 2006, 15(2): 303-306(in Chinese). 聂发辉. 镉超富集植物商陆及其富集效应
[J]. 生态环境, 2006, 15(2): 303-306.

[3] Cosgrove D J. Characterization of long-term extension of isolated cell walls from growing cucumber hypocotyls
[J]. Planta, 1989, 177: 121-130.

[4] Chen A G, Chen J H. Advances in the study of expansin
[J]. Chinese Bulletin of Botany, 2003 , 20(6): 752-758(in Chinese). 陈爱国, 陈进红. Expansin 研究进展
[J]. 植物学通报, 2003 , 20(6): 752-758.

[5] Chen F, Dahal P, Bradford K J. Two tomato expansin genes show divergent expression and localization in embryos during seed development and germination
[J]. Plant Physiology, 2001, 127: 928-936.

[6] Yang H Q, Jie Y L, Huang T D, et al. The induction of root formation by urea, IBA and sheep dung in young apple tree
[J]. Science Agricultura Sinica, 2001, 34(1): 51-55(in Chinese). 杨洪强, 接玉玲, 黄天栋, 等. 尿素、IBA和羊粪对苹果幼树新根的诱导与调控
[J]. 中国农业科学, 2001, 34(1): 51-55.

[7] Shin J H, Jeong D H, Park M C, et al. Characterization and transcriptional expression of the α-expansin gene family in rice
[J].Molecules and Cells, 2005, 20: 210-218.

[8] Keller E, Cosgrove D J. Expansins in growing tomato leaves
[J]. Plant Journal, 1995, 8: 795- 802.

[9] Civello P M, Powell A LT, Sabehat A, et al. An expansin gene expressed in ripening strawberry fruit
[J]. Plant Physiology, 1999, 121: 1273-1279.

[10] Cosgrove D J. Loosening of plant cell walls by expansins
[J]. Nature, 2000, 407: 321-326.

[11] 亚当斯, 戈特施林, 凯泽特, 等. 酵母遗传学方法实验指南
[M]. 北京:科学出版社, 2000: 181-821.

[12] Wu Y, Sharp R E, Cosgrove D J.Growth maintenance of the maize primary root at low water potentials involves increases in cell extension properties Expansins activity and wall susceptibility to Expansins
[J]. Plant Physiology, 1996, 111: 765-772.

[13] Zhao P, Chen S, Wang X C. Arabidopsis expansin AtEXP1 involved in the regulation of stomatal movement
[J]. Acta Agronomica Sinica. 2006, 3(4): 562-567(in Chinese). 赵萍, 陈苏, 王学臣. 拟南芥扩张蛋白AtEXP1参与气孔运动的调控
[J]. 作物学报, 2006, 3(4): 562-567.

[14] Cosgrove D J. Enzymes and other agents that enhance cell wall extensibility //Annu Rev Plant Physiol Plant Mol Biol, 1999, 50: 391-417.