Construction of RNAi and root-specific expression vectors of genes related to low Cd accumulation in rice

doi:10.7523/j.issn.2095-6134.2015.02.006

Abstract

Abstract:

Cd accumulated in soil can be easily absorbed by rice and enriched in the human body through food chain. Investigation of the mechanism for the low Cd accumulation in rice has an important practical value for standard rice breeding and safety of food crops. Multiple genes are involved in the process of the absorption, transport, and accumulation of Cd in rice. The technique of multi-gene co-transformation facilitates the genetic control of low Cd accumulation in rice grain. We cloned two genes that are related to this process, i.e., OsLCD and OsHMA 3 , and a vascular-specific promoter OsMTP 11 P. The RNAi vector pRI-M-LCD and the root-specific expression vector pCB2022-H were constructed by using the gateway technology and traditional enzyme digestion and ligation methods. Through Agrobacterium tumefaciens-mediated transformation, the transgenic rice plants containing both the two transgenes are expected to sequester more Cd into the root vacuoles and to reduce the root-to-shoot translocation of Cd, resulting in low Cd accumulation in grains. The successful construction of the two vectors will be useful in molecular regulation of low Cd accumulation in rice and will lay the foundation for breeding new varieties of rice that accumulates lower Cd in grains.

Key words: rice, RNA interference, tissue-specific expression, low-Cd accumulation, co-transformation, vector construction

CLC Number:

S511

CHEN Yuxian, FENG Shanshan, CHAI Xingping, CHAI Tuanyao. Construction of RNAi and root-specific expression vectors of genes related to low Cd accumulation in rice[J]. , 2015, 32(2): 185-191.

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