Isolation and expression of heavy metal ATPase in Brassica juncea L.

doi:10.7523/j.issn.2095-6134.2011.4.017

Abstract

Abstract:

Brassica juncea L. is a Zn/Cd tolerance plant, and the gene expression of heavy metal ATPase (HMA) has not been reported. In the present work, two cDNA fragments of BjHMA, BjHMA 3 and BjHMA 4 , were isolated from B. juncea seedling. Real-time quantitative PCR analysis revealed that both BjHMA 3 and BjHMA 4 were constitutively expressed in all tissue and preferentially in root. The mRNA profile of BjHMA 3 or BjHMA 4 was the lowest in leaf while the expressions were strongly enhanced by Zn or Cd stress, indicating that BjHMA played important roles in growth and in heavy metal homeostasis and tolerance in plant.

Key words: Brassica juncea L., heavy metal ATPase, gene expression

CLC Number:

Q942.6

ZHANG Yu-Xiu, ZHANG Yuan-Ya, CHAI Tuan-Yao. Isolation and expression of heavy metal ATPase in Brassica juncea L.[J]. , 2011, 28(4): 551-555.

References

[1] Clemens S. Molecular mechanisms of plant metal tolerance and homeostasis
[J]. Planta, 2001, 212(4): 475-486.

[2] Zhang Y X, Chai T Y. Isolation and function of heavy metal responsive gene in plant
[M]. Beijing: China Agr Press, 2006: 26-34. 张玉秀, 柴团耀. 植物重金属调节基因的分离和功能
[M]. 北京: 中国农业出版社, 2006: 26-34.

[3] Mills R F. Krijger G C, Baccarini P J, et al. Functional expression of AtHMA4, a P1B-type ATPase of the Zn/Co/Cd/Pb subclass
[J]. Plant J, 2003, 35(2): 164-176.

[4] Palmgren M G, Clemens S, Williams L E, et al. Zinc biofortification of cereals:problems and solutions
[J]. Trends Plant Sci, 2008, 13(9): 464-473.

[5] Mills R F, Francini A, Pedro S C, et al. The plant P_1B-type ATPase AtHMA4 transports Zn and Cd and plays a role in detoxification of transition metals supplied at elevated levels
[J]. FEBS Lett, 2005, 579(3): 783-791.

[6] Clemens S, Palmgren M G, Krämer U. A long way ahead: understanding and engineering plant metal accumulation
[J]. Trends Plant Sci, 2002, 7(7): 309-315.

[7] Papoyan A, Kochian L V. Identification of Thlaspi caerulescen genes that may be involved in heavy metal hyperaccumulation and tolerance. characterization of a novel heavy metal transporting ATPase
[J]. Plant Physiol, 2004, 36(11): 3814-3823.

[8] Bernard C, Roosens N, Czernic P, et al. A novel CPx-ATPase from the cadmium hyperaccumulator Thlaspi caerulescens
[J]. FEBS Lett, 2004, 569(1-3): 140-148.

[9] Courbot M, Willems G, Motte P, et al. A major quantitative trait locus for cadmium tolerance in Arabidopsis halleri colocalizes with HMA4, a gene encoding a heavy metal ATPase
[J]. Plant Physiol, 2007, 144(2): 1052-1065.

[10] Hanikenne M, Talke I N, Haydon M J. Evolution of metal hyperaccumulation required cis-regulatory changes and triplication of HMA4
[J]. Nature, 2008, 453(7139): 391-396.

[11] Verret F, Gravot A, Auroy P, Leonhardt N, et al. Overexpression of AtHMA4 enhances root-to-shoot translocation of zinc and cadmium and plant metal tolerance
[J]. FEBS Lett,2004, 576: 306-312.

[12] Morel M, Crouzet J. AtHMA3, a P1B-ATPase allowing Cd/Zn/Co/Pb vacuolar storage in Arabidopsis
[J]. Plant Physiol, 2009, 149(2): 894-904.

[13] Becher M, Talke I N, Krall L. Cross-species microarray transcript profiling reveals high constitutive expression of metal homeostasis genes in shoots of the zinc hyperaccumulator Arabidopsis halleri
[J]. Plant J, 2004, 37(2): 251-268.

[14] Morin I, Gudin S, Mintz E, et al. Dissecting the role of the N-terminal metal-binding domains in activating the yeast copper ATPase in vivo
[J]. FEBS J, 2009, 276(16): 4483-4495.