Cloning and expression of PaOPT in Phytolacca americana

doi:10.7523/j.issn.2095-6134.2012.3.006

Abstract

Abstract: An oligopeptide transporter gene was isolated from Cd-treated leaf tissue of Phytolacca americana by SSH method, designated by PaOPT (GenBank accession No. HQ647015). The full length of this gene has been cloned by race-PCR technique. Moreover, the evolution of PaOPT in OPT family and the expression profiles during seed germination period was investigated. Our study indicates that PaOPT belongs to the OPT3 family. The results of real-time quantitative PCR show that PaOPT may be a seed-specific expression gene. Further investigation of the function and localization of PaOPT will be favorable for understanding of plant growth and development.

Key words: Phytolacca Americana, PaOPT, expression

CLC Number:

Q945.3

JIN Li, ZHENG Hong-Chun, CHAI Tuan-Yao. Cloning and expression of PaOPT in Phytolacca americana[J]. , 2012, (3): 316-323.

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