Analysis of glutathione in cells by microchip electrophoresis coupled with electrochemical detection

doi:10.7523/j.issn.2095-6134.2014.01.007

Abstract

Abstract:

We established a new method for simultaneous determination of reduced and oxidized glutathione. The separation and detection conditions were optimized in terms of the buffer pH, buffer concentration, SDS concentration, separation potential, injection time, and detection potential. Under the optimized condition, GSH and GSSG were separated within 3 min, and the satisfactory linear ranges were 5~200 and 2~100 μmol/L for GSH and GSSG, respectively. The detection limits of GSH and GSSG were 4.87 and 1.98 μmol/L, respectively. Quantitative analyses of GSSG and GSH in cells were performed with satisfactory results.

Key words: alloy modified electrode, glutathione, microchip electrophoresis, electrochemical detection

CLC Number:

O658

YAO Lin, WU Liangqi, DING Yongsheng. Analysis of glutathione in cells by microchip electrophoresis coupled with electrochemical detection[J]. , 2014, 31(1): 38-45.

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