Effects of nitric oxide on the biosynthesis of shikonin and its derivatives in callus of Arnebia euchroma

doi:10.7523/j.issn.2095-6134.2014.01.008

Abstract

Abstract:

Nitric oxide (NO) is an important signal molecule involved in the regulation of plant secondary metabolism. In this study, SNP (sodium nitroprusside) was utilized as the donor of nitric oxide to treat Arnebia euchroma callus. We find that the expression levels of the genes involved in the biosynthesis of shikonin and its derivatives, such as AePGT, AeGPPS, AeC4H, and Ae4CL, increase significantly in SNP-treated Red Strain callus tissues. Meanwhile, SNP treatment also increases the contents of some shikonin derivatives. The above-mentioned results indicate that exogenous NO increases the biosynthesis of shikonin and its derivatives by up-regulating the expression of the genes.

Key words: Arnebia euchroma, nitric oxide, shikonin and its derivatives, sodium nitroprusside, gene expression

CLC Number:

S567.239

HAO He, YE Hechun, WANG Hong. Effects of nitric oxide on the biosynthesis of shikonin and its derivatives in callus of Arnebia euchroma[J]. , 2014, 31(1): 46-53.

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