Anaerobic ammonium-oxidazing bacteria community compositions in different reactors and sludge morphologies

doi:10.7523/j.issn.2095-6134.2014.01.011

Abstract

Abstract:

In this study, all the dominant anammox bacteria were distributed over two genera, Candidatus Brocadia and Kuenenia, in four anammox reactors. It was shown that sludge morphology played an important role in the bacterial community structure of anammox bacteria. More than 36 percents of anammox bacteria in granular sludge and biofilm belonged to Candidatus Brocadia, while in flocculent sludge the domninant anammmox bacteria was Candidatus Kuenenia. Anammox bacteria in different sludge morphologies would migrate in the same reactor, but the dominant species remained.

Key words: anammox bacteria, granular, flocculent, biofilm, PCR-clone

CLC Number:

X172

XU Yahui, LIU Miaomiao, ZHANG Shujun, ZHANG Zhennan, LI Juan, LIU Xinchun. Anaerobic ammonium-oxidazing bacteria community compositions in different reactors and sludge morphologies[J]. Journal of University of Chinese Academy of Sciences, 2014, 31(1): 67-73.

References

[1] 郑平, 徐向阳, 胡宝兰. 新型生物脱氮理论与技术[M]. 北京:科学出版社, 2004.

[2] Mulder A, Vandegraaf A A, Robertson L A, et al. Anaerobic ammonium oxidation discovered in a denitrifying fluidized-bed reactor[J]. Fems Microbiology Ecology, 1995, 16(3): 177-183.

[3] Strous M, Heijnen J J, Kuenen J G, et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J]. Applied Microbiology and Biotechnology, 1998, 50(5): 589-596.

[4] Trigo C, Campos J L, Garrido J M, et al. Start-up of the anammox process in a membrane bioreactor[J]. Journal of Biotechnology, 2006, 126(4): 475-487.

[5] Jetten M S M, Horn S J, VanLoosdrecht M C M. Towards a more sustainable municipal wastewater treatment system[J]. Water Science and Technology, 1997, 35(9): 171-180.

[6] Van Niftrik L A, Fuerst J A, Damste J S S, et al. The anammoxosome: an intracytoplasmic compartment in anammox bacteria[J].Fems Microbiology Letters, 2004, 233(1):7-13.

[7] vande Vossenberg J, Rattray J E, Geerts W, et al. Enrichment and characterization of marine anammox bacteria associated with global nitrogen gas production[J]. Environmental Microbiology, 2008, 10(11): 3120-3129.

[8] Schmid M C, Risgaard-Petersen N, vande Vossenberg J, et al. Anaerobic ammonium-oxidizing bacteria in marine environments: widespread occurrence but low diversity[J]. Environmental Microbiology, 2007, 9(6): 1476-1484.

[9] Quan Z X, Rhee S K, Zuo J E, et al. Diversity of ammonium-oxidizing bacteria in a granular sludge anaerobic ammonium-oxidizing (anammox) reactor[J]. Environmental Microbiology, 2008, 10(11): 3130-3139.

[10] Penton C R, Devol A H, Tiedje J M. Molecular evidence for the broad distribution of anaerobic ammonium-oxidizing bacteria in freshwater and marine sediments[J]. Applied and Environmental Microbiology, 2006, 72(10): 6829-6832.

[11] Kartal B, Rattray J, Van Niftrik L A, et al. Candidatus "anammoxoglobus propionicus" a new propionate oxidizing species of anaerobic ammonium oxidizing bacteria[J]. Systematic and Applied Microbiology, 2007, 30(1): 39-49.

[12] Lam P, Lavik G, Jensen M M, et al. Revising the nitrogen cycle in the Peruvian oxygen minimum zone[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(12): 4752-4757.

[13] Kawagoshi Y, Nakamura Y, Kawashima H, et al. Enrichment culture of marine anaerobic ammonium oxidation (anammox) bacteria from sediment of sea-based waste disposal site[J]. Journal of Bioscience and Bioengineering, 2009, 107(1): 61-63.

[14] Kartal B, Koleva M, Arsov R, et al. Adaptation of a freshwater anammox population to high salinity wastewater[J]. Journal of Biotechnology, 2006, 126(4): 546-553.

[15] Lopez H, Puig S, Ganigue R, et al. Start-up and enrichment of a granular anammox SBR to treat high nitrogen load wastewaters[J]. Journal of Chemical Technology and Biotechnology, 2008, 83(3): 233-241.

[16] Sanchez-Melsio A, Caliz J, Balaguer M D, et al. Development of batch-culture enrichment coupled to molecular detection for screening of natural and man-made environments in search of anammox bacteria for N-removal bioreactors systems[J]. Chemosphere, 2009, 75(2): 169-179.

[17] Wang H, Yi P, Zhang S J, et al. The functional microbial community composition analysis in the laboratory-scale stable partial nitrifying-anammox municipal wastewater reactor[J]. Journal of Graduate University of Chinese Academy of Sciences, 2012, 29(1):38-46(in Chinese). 王惠, 易鹏, 张树军, 等. 短程硝化-anammox反应器处理城市生活污水过程中脱氮微生物的群落解析[J].中国科学院研究生院学报, 2012, 29(1): 38-46.

[18] Vlaeminck S E, Terada A, Smets B F, et al. Aggregate size and architecture determine microbial activity balance for one-stage partial nitritation and anammox[J]. Applied and Environmental Microbiology, 2010, 76(3): 900-909.

[19] Kartal B, Van Niftrik L, Rattray J, et al. Candidatus‘brocadia fulgida': an autofluorescent anaerobic ammonium oxidizing bacterium[J]. Fems Microbiology Ecology, 2008, 63(1): 46-55.

[20] Hu B L, Zheng P, Tang C J, et al. Identification and quantification of anammox bacteria in eight nitrogen removal reactors[J]. Water Research, 2010, 44(17):5014-5020.

[21] Wang Y H, Ma Y X, Liu C F, et al. The bacterial diversity in biofilms associated with media of anammox bioreactors[J]. Journal of Dalian Ocean University, 2011, 26(6):500-506(in Chinese). 王银华, 马悦欣, 刘长发, 等. 厌氧氨氧化反应器载体生物膜细菌多样性的研究[J]. 大连海洋大学学报, 2011, 26(6):500-506.

[22] Wang H, Liu Y P, Tao Y, et al. The biochemical mechanism and application of anammox in the wastewater treatment process[J]. Acta Ecologica Sinica, 2011, 31(7):2019-2028(in Chinese).王惠, 刘研萍, 陶莹, 等. 厌氧氨氧化菌脱氮机理及其在污水处理中的应用[J]. 生态学报, 2011, 31(7): 2019-2028.

[23] Wang T, Zhang H M, Gao D W, et al. Enrichment of anammox bacteria in seed sludges from different wastewater treating processes and start-up of anammox process[J]. Desalination, 2011, 271(1-3): 193-198.

[24] Innerebner G, Insam H, Franke-Whittle I H, et al. Identification of anammox bacteria in a full-scale deammonification plant making use of anaerobic ammonia oxidation[J]. Systematic and Applied Microbiology, 2007, 30(5): 408-412.

[25] Xiao Y, Yang Z H, Zeng G M, et al. Bacterial diversity in sequencing batch biofilm reactor (SBBR) for landfill leachate treatment using PCR-DGGE[J]. Enviromental Science, 2007, 28(5): 1095-1101(in Chinese). 肖勇, 杨朝晖, 曾光明, 等. PCR-DGGE研究处理垃圾渗滤液序批式生物膜反应器(SBBR)中的细菌多样性[J]. 环境科学, 2007, 28(5): 1095-1101.

[26] Chen T T, Zheng P, Shen L D, et al. Dispersal and control of anammox granular sludge at high substrate concentrations[J]. Biotechnology and Bioprocess Engineering, 2012, 17(5): 1093-1102.

[27] Ni S Q. Study of anammox process performance and microbial characteristic. Jinan: Shandong University, 2010(in Chinese). 倪寿清. 厌氧氨氧化过程性能和微生物特性研究[D]. 济南: 山东大学, 2010.

[28] Cirpus I E Y, Geerts W, Hermans J H M, et al. Challenging protein purification from anammox bacteria[J]. International Journal of Biological Macromolecules, 2006, 39(1-3): 88-94.

[29] Jetten M S M, Van Niftrik L, Strous M, et al. Biochemistry and molecular biology of anammox bacteria[J]. Critical Reviews in Biochemistry and Molecular Biology, 2009, 44(2/3): 65-84.