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›› 2004, Vol. 21 ›› Issue (4): 488-493.DOI: 10.7523/j.issn.2095-6134.2004.4.010

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Characterization of13C2Labeled Uridine Diphosphoglucoseof Enzymatic Synthesis by NMR

QI Chao1,2, LIU Li-Yan2   

  1. 1. Key Laboratory of Structural Biology, School of Life Science, University of Science and Technology of China, Hefei 230027, China;
    2. Key Laboratory of Chemistry and Physics of Rare Earth, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Online:2004-07-10

Abstract:

The authors set up enzymatic method of one step to synthesize UDPG in much higher yields, which iseconomical and cheap. In enzymatic synthesis, UTP is used to replace ATP, and repetitive system of UTP is setup. Repetitive addition and recovery of enzyme are used to take advantage of enzyme. UDP-[4-13C]-glucose are firstsynthesized on 0.5 g scale by this method. The overall yield is 69.7 %. NMR spectra of 13C-labelled UDPGmakecertain the assignment of C-4 of the glucose residue and suggest that part of the results in the literatures about theassignment of UDPG are wrong

Key words: UDPG, enzymatic synthesis, enzymatic method of one step, NMR

CLC Number: