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›› 2002, Vol. 19 ›› Issue (4): 436-442.DOI: 10.7523/j.issn.2095-6134.2002.4.017

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Quantitation of Conjugated Linoleic Acid by UV Spectrophotometry

ZHANG Ya Gang1, 2, FAN Li1, 2, MA Li2, WEN Bin2, Wumanjiang·ELI2   

  1. 1. Graduate School of Chinese Academy of Sciences, Beijing 100039;
    2. Xinjiang Institute of Physical and Chemical Technology, Chinese Academy of Sciences, Urumqi 830011
  • Received:2002-01-13 Revised:2002-04-24 Online:2002-07-18

Abstract:

Through absorbance measurement of conjugated linoleic acid, methy lester of conjugated linoleic acid and conjug ated linoleic acid trigly cide from 210 ~ 300 nm by UV-9100 spectrophotometer, absorbancy curve of measured samples at different concent ration is plotted.In the appropriate concentration range of samples, linear relationship of absorbancy and samples concent ration is presented by linear regression with Microcal Software Origin Version 4.0 system.The accurate concentration of conjugated linoleic acid is obtained with PE Autosystem XL-T ruboMass.Combined w ith reg ression constant data, emprical formula for the quantitation of conjugated linoleic acid by UV spect rophotometer is given.

Key words: ult raviolet-visible spect rophotometry, conjugated linoleic acid, octadecadienoic acid

CLC Number: