Welcome to Journal of University of Chinese Academy of Sciences,Today is

Previous Articles     Next Articles

Phylogenetic relationships among ten Elymus species based on random amplified polymorphic DNA

ZHOU Yong-Hong, ZHENG You-Liang, YANG Jun-Liang, YAN Ji, JIA Ji-Zeng   

  • Received:1900-01-01 Revised:1900-01-01 Online:1999-09-10 Published:1999-09-10
  • Contact: ZHOU Yong-Hong

Abstract:

Ten species of Elymus (Poaceae: Triticeae) were analyzed using random ampli-
lied polymorphic DNA (RAPD) markers.  Thirty-four decamer oligonucleotide random
primers from Opron Technologies were used for polymorphic selection. 25 (73.53%) pro-
duced polymorphic products. A total of 136 bands amplified from 16 primers were selected
for RAPD analyses. The data were used to generate Nei's similarity coefficients and to con-
struct a dendrogram using UPGMA in NTSYS programs. The result showed that: (1)
Three tetraploid species, E. sibiricus L., E. caninus (L.) L. and E. lanceolatus (Scrib-
ner et Smith) Gould, were clustered in one group, while seven hexaploid species, E. nu-
tans Griseb., E. dahuricusTurcz., E. brachyaristatus A Löve, E. submuticus (Keng)
Keng f., E. tangutorum (Nevski)Hand.-Mazz., E. excelsus Turcz. and E. cylindricus
(Franch) Honda, were clustered in another group. The relationship between the tetraploid
and the hexaploid species was remote; (2) E. caninus was closely related to E. sibiricus.
This result supported that Roegneria canina (L.) Nevski was treated as Elymus caninus;
(3) E . nutans was closely related to E. dahuricus ; (4) E.brachyaristatus and E.sub-
muticus were morphologically similar and sympatric in distribution, but there were certain
differences between them in the nucleotide sequences. Both of them were related to E. nu-
tans and E. duhuricus; (5) E. excelsus was closely related to E. cylindricus, and they
were clustered with E. tangutorum; (6) RAPD results are basically comparable with those
obtained from studies on morphology and cytology. It is a useful supplementary method for
assessing the genetic relationships among Elymus species.

Key words: Elymus, RAFD ahalysis, Phylogenetic relationship, Genome, Morphology