Combination extracting main functional protein such as thrombin and immunoglobulin from pig plasma

LI Min-Kang; QIAN Dong-Ming; SONG Hong-Xin

doi:10.7523/j.issn.2095-6134.2007.3.014

Journal of University of Chinese Academy of Sciences >

2007 , Vol. 24 >Issue 3: 357 - 361

DOI: https://doi.org/10.7523/j.issn.2095-6134.2007.3.014

简报

Combination extracting main functional protein such as thrombin and immunoglobulin from pig plasma

LI Min-Kang ,
QIAN Dong-Ming ,
SONG Hong-Xin

Expand

College of Life Science and Engineering, Shaanxi University of Science & Technology Xianyang, Shaanxi 712081

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2007-05-15

Fold

Abstract

A effective method of extracting functionality protein such as albumin、immunoglobulin、fibrinogen and thrombin from pig plasma was introduced in this article. Extracting fibrinogen with freeze-thawing method and thrombin from supernatant by absorbing test, then salt out to distill albumin and immunoglobulin. In 1L pig plasma ，about 31.41g albumin and 13.86g immunoglobulin and 0.22g thrombin and 2.67g fibrinogen have been extracted. The specific activity of thrombin is 38.5U/mg.

Key words： functional protein ；freeze thawing ；adsorption；salt out

Cite this article

LI Min-Kang , QIAN Dong-Ming , SONG Hong-Xin . Combination extracting main functional protein such as thrombin and immunoglobulin from pig plasma[J]. Journal of University of Chinese Academy of Sciences, 2007 , 24(3) : 357 -361 . DOI: 10.7523/j.issn.2095-6134.2007.3.014

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article