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Purification and characterization of β-glucosidase from Aspergillus niger
Received date: 2008-07-25
Revised date: 2008-10-23
Online published: 2009-03-15
A high activity glucosidase, which can hydrolyze glycosides, was isolated from Aspergillus niger S strain. The enzyme was purified to one spot after ammonium sulfate precipitation, ion exchange & size exclusion chromatography and its molecular weight was about 165ku, which was quite differ from other reported glucosidase. The optimum temperature of this enzyme was 50℃ & the optimum pH was pH 5~10. Based on the HPLC result, this enzyme can transform glycone to aglycone of isoflavones & resveratrol with high specific activity.
Key words： soybean isoflavones; resveratrol; glucosidase; purification; characterization
WANG Yun , ZENG Wo-Tan . Purification and characterization of β-glucosidase from Aspergillus niger[J]. Journal of University of Chinese Academy of Sciences, 2009 , 26(2) : 274 -279 . DOI: 10.7523/j.issn.2095-6134.2009.2.018
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