Journal of University of Chinese Academy of Sciences >
Recognition and detection of target DNA using radio-labelled peptide nucleic acids
Received date: 2011-11-09
Revised date: 2012-02-17
Online published: 2013-01-15
The radio-111In3+ labelled peptide nucleic acid (PNA) was used as molecular probe and the pUC19-C1 plasmid which contains the target site of the PNA probe was constructed. The results show that PNA forms a stable double helix with not only the complementary single-stranded nucleic acid but also the plasmid in the presence of PNA opener. It is suggested that radio-labelled PNA probe can be used to detect target DNA in genes.
WANG Guang-Wei , HUANG Liang , XIANG Kai-Xiang , ZHAO Hong , HE Yu-Jian . Recognition and detection of target DNA using radio-labelled peptide nucleic acids[J]. Journal of University of Chinese Academy of Sciences, 2013 , 30(1) : 1 -5 . DOI: 10.7523/j.issn.1002-1175.2013.01.001
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