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›› 2013, Vol. 30 ›› Issue (1): 1-5.DOI: 10.7523/j.issn.1002-1175.2013.01.001

• Research Articles •     Next Articles

Recognition and detection of target DNA using radio-labelled peptide nucleic acids

WANG Guang-Wei1, HUANG Liang1, XIANG Kai-Xiang2, ZHAO Hong3, HE Yu-Jian3,4   

  1. 1. Medical College, Hunan Normal University, Changsha 410006, China;
    2. Huaihua Medical College, Huaihua 418000, Hunan, China;
    3. College of Chemistry and Chemical Engineering, Graduate University, Chinese Academy of Sciences, Beijing 100049, China;
    4. State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, China
  • Received:2011-11-09 Revised:2012-02-17 Online:2013-01-15

Abstract:

The radio-111In3+ labelled peptide nucleic acid (PNA) was used as molecular probe and the pUC19-C1 plasmid which contains the target site of the PNA probe was constructed. The results show that PNA forms a stable double helix with not only the complementary single-stranded nucleic acid but also the plasmid in the presence of PNA opener. It is suggested that radio-labelled PNA probe can be used to detect target DNA in genes.

Key words: peptide nucleic acid, radio-labelled, PNA opener, sequence specifically binding

CLC Number: