Journal of University of Chinese Academy of Sciences >
Detection of selected pathogenic bacteria during oxidation ditch wastewater treatment
Received date: 2012-01-13
Revised date: 2012-03-27
Online published: 2013-01-15
Indicator bacterium E. coli and pathogenic bacteria Salmonella and Legionella were detected during the wastewater treatment of oxidation ditch, using SYBR I Real-time quantitative PCR. The detected amount of E.coli was 108 copies/mL and decreased by 2 orders of magnitude eventually. In the initial stages, the amount of Salmonella ranged from 102 to 103 copies/mL but was eliminated or reduced to undetectable level eventually. The amount of Legionella was detected to be 10-102 copies/mL in the sample of activated sludge. However, the amount of Legionella in the effluent samples was detectable and similar to those in the influent samples, which ranged from 104 to 105 copies/mL.
Key words: oxidation ditch; pathogenic bacteria; real-time quantitative PCR
LIN Yu-Xin , ZHANG Zhen-Nan , XU Ya-Hui , LI Juan , LIU Xin-Chun . Detection of selected pathogenic bacteria during oxidation ditch wastewater treatment[J]. Journal of University of Chinese Academy of Sciences, 2013 , 30(1) : 40 -46 . DOI: 10.7523/j.issn.1002-1175.2013.01.007
[1] S Meric D F K. Water treatment, municipal, encyclopedia of microbiology[M]. 2009: 587-599.
[2] Groisman E. Principles of bacterial pathogenesis[M]. Academic Press, 2000.
[3] Wéry N, Lhoutellier C, Ducray F, et al. Behaviour of pathogenic and indicator bacteria during urban wastewater treatment and sludge composting, as revealed by quantitative PCR[J]. Water Research, 2008, 42(1-2): 53-62.
[4] Sinclair R G, Jones E L, Gerba C P. Viruses in recreational water-borne disease outbreaks: a review[J]. Journal of Applied Microbiology, 2009, 107(6): 1769-1780.
[5] Mcfeters G A, Bissonnette G K, Jezeski J J, et al. Comparative survival of indicator bacteria and enteric pathogens in well water[J]. Applied and Environmental Microbiology, 1974, 27(5): 823.
[6] Pote J, Haller L, Kottelat R, et al. Persistence and growth of faecal culturable bacterial indicators in water column and sediments of Vidy Bay, Lake Geneva, Switzerland[J]. Journal of Environmental Sciences, 2009, 21(1): 62-69.
[7] Field K G, Bernhard A E, Brodeur T J. Molecular approaches to microbiological monitoring: fecal source detection[J]. Environmental Monitoring and Assessment, 2003, 81(1): 313-326.
[8] Savichtcheva O, Okabe S. Alternative indicators of fecal pollution: relations with pathogens and conventional indicators, current methodologies for direct pathogen monitoring and future application perspectives[J]. Water Research, 2006, 40(13): 2463-2476.
[9] Zuo L L. Study on the application of the detection of Pathogen in environmental water by quantitative PCR . Xi'an:Xi'an University of Architecture and Technology, 2008(in Chinese). 左丽丽. 定量 PCR 技术在环境水体病原细菌检测中的应用研究 . 西安:西安建筑科技大学, 2008.
[10] Nazarian E J, Bopp D J, Saylors A, et al. Design and implementation of a protocol for the detection of Legionella in clinical and environmental samples[J]. Diagnostic Microbiology and Infectious Disease, 2008, 62(2):125-132.
[11] Tsushima I, Ogasawara Y, Kindaichi T, et al. Development of high-rate anaerobic ammonium-oxidizing (anammox) biofilm reactors[J]. Water Research, 2007, 41(8): 1623-1634.
[12] Watanabe K, Kodama Y, Harayama S. Design and evaluation of PCR primers to amplify bacterial 16S ribosomal DNA fragments used for community fingerprinting[J]. Journal of Microbiological Methods, 2001, 44(3): 253-262.
[13] Maheux A F, Picard F J, Boissinot M, et al. Analytical comparison of nine PCR primer sets designed to detect the presence of Escherichia coli/Shigella in water samples[J]. Water Research, 2009, 43: 3019-3028.
[14] Tantawiwat S, Tansuphasiri U, Wongwit W, et al. Development of multiplex PCR for the detection of total coliform bacteria for Escherichia coli and Clostridium perfringens in drinking water[J]. Southeast Asian J Trop Med Public Health, 2005, 36(1):162-169.
[15] Rahn K, De Grandis S A, Clarke R C, et al. Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella[J]. Molecular and Cellular Probes, 1992, 6(4): 271-279.
[16] Miyamoto H, Yamamoto H, Arima K, et al. Development of a new seminested PCR method for detection of Legionella species and its application to surveillance of legionellae in hospital cooling tower water[J]. Applied and Environmental Microbiology, 1997, 63(7): 2489-2494.
[17] Godfree A, Farrell J. Processes for managing pathogens[J]. J Environ Qual, 2005, 34(1): 105-113.
[18] Garcia-Armisen T, Servais P. Respective contributions of point and non-point sources of E. coli and enterococci in a large urbanized watershed (the Seine river, France)[J]. Journal of Environmental Management, 2007, 82(4): 512-518.
[19] Gantzer C, Gaspard P, Galvez L, et al. Monitoring of bacterial and parasitological contamination during various treatment of sludge[J]. Water Research, 2001, 35(16): 3763-3770.
[20] Sahlström L, Aspan A, Bagge E, et al. Bacterial pathogen incidences in sludge from Swedish sewage treatment plants[J]. Water Research, 2004, 38(8): 1989-1994.
[21] Zeng S, Zhang C M, Wang X C, et al. PCR detection of Salmonella in secondary effluent in municipal wastewater treatment system[J]. Research of Environmental Sciences, 2010, 23(3):361-365(in Chinese). 曾颂,张崇淼,王晓昌,等.城市污水二级处理出水中沙门氏菌的 PCR 检测[J].环境科学研究, 2010,23(3): 361-365.
[22] Wadowsky R M, Wolford R, Mcnamara A M, et al. Effect of temperature, pH, and oxygen level on the multiplication of naturally occurring Legionella pneumophila in potable water[J]. Applied and Environmental Microbiology, 1985, 49(5): 1197.
[23] Wullings B A, Van Der Kooij D. Occurrence and genetic diversity of uncultured legionella spp in drinking water treated at temperatures below 15 {degrees} C[J]. Applied and Environmental Microbiology, 2006, 72(1): 157.
[24] Sheehan K B, Henson J M, Ferris M J. Legionella species diversity in an acidic biofilm community in Yellowstone National park[J]. Applied and Environmental Microbiology, 2005, 71(1): 507-511.
[25] Winfield M D, Groisman E A. Role of nonhost environments in the lifestyles of Salmonella and Escherichia coli[J]. Applied and Environmental Microbiology, 2003, 69(7): 3687-3694.
[26] Shannon K E, Lee D Y, Trevors J T, et al. Application of real-time quantitative PCR for the detection of selected bacterial pathogens during municipal wastewater treatment[J]. Science of the Total Environment, 2007, 382(1): 121-129.
/
| 〈 |
|
〉 |