Journal of University of Chinese Academy of Sciences >
Involvement of AtACO3 in Zn homeostasis
Received date: 2012-03-16
Revised date: 2012-04-28
Online published: 2012-04-28
A full-length cDNA, designated as AtACO3, was isolated from Arabidopsis thaliana. Semi-quantitative RT-PCR revealed that AtACO3 was significantly activated under treatment with 200 μmol/L ZnCl2 for 1 hour while inhibited under treatment with 200 μmol/L CuCl2. To investigate the function of the N-terminus of AtACO3, the prokaryotic expression vector pET30a with a fragment of AtACO3 including 1632 bp from the initiation codon was constructed and successfully transformed E. coli. SDS-PAGE indicated that the recombinant N-terminus of AtACO3 was soluble and stable when expressed in E. coli. Additionally, the tolerance of E. coli against Zn2+ was found to be enhanced by expressing the N-terminus fragment of AtACO3.
Key words： Zn; Arabidopsis thaliana; aconitase
LI Yan , WANG Jian-Wu , TAN Jin-Juan , FENG Shan-Shan , CHAI Tuan-Yao . Involvement of AtACO3 in Zn homeostasis[J]. Journal of University of Chinese Academy of Sciences, 2013 , 30(3) : 329 -333 . DOI: 10.7523/j.issn.1002-1175.2013.03.008
[1] Jordanov J, Courtois-Verniquet F, Neuburger M, et al. Structural investigation by extended X-ray absorption fine structure spectroscopy of the iron center of mitochondrial aconitase in higher plant cells[J]. J Biol Chem, 1992, 267:16775-16778.
[2] Brouquisse R, Nishimura M, Gaillard J, et al. Characterization of a cytosolic aconitase in higher plant cell[J]. Plant Physiol, 1987, 84(4):1402-1407.
[3] Zhang W L, Shen W B, Ye M B, et al. Aconitase and its physiological roles in plant[J]. Plant Physiology Communications, 2003, 39(4):391-398 (in Chinese). 张文利,沈文飚, 叶茂炳,等. 植物顺乌头酸酶及其生理功能[J]. 植物生理学通讯, 2003, 39(4):391-398.
[4] Hentze M W, Kuhn L C. Molecular control of vertebrate iron metabolism: mRNA-based regulatory circuits operated by iron, nitricoxide, and oxidative stress[J]. Proc Natl Acad Sci USA, 1996, 193:8175-8182.
[5] Kennedy M C, Emptage M H, Dreyer J L, et al. The role of iron in the activation-inactivation of aconitase[J]. J Biol Chem, 1983, 258(18):11098-11105.
[6] Beinert H, Kennedy M C, Stout D C. Aconitase as iron-sulfur protein, enzyme, and iron-regulatory protein[J]. Chem Rev, 1996, 96:2335-2373.
[7] Arnaud N, Ravet K, Borlotti A, et al. The iron responsive element (IRE)/iron regulatory protein1 (IRP1)-cytosolic aconitase iron regulatory switch does not operate in plants[J]. Biochem J, 2007, 405:523-531.
[8] Peyret P, Perez P, Alric M. Structure genomic organization and expression of the Arabidopsis thaliana aconitase gene[J]. J Biol Chem, 1995, 270:8131-8137.
[9] Moeder W, Del Pozo O, Navarre D A, et al. Aconitase plays a role in regulating resistance to oxidative stress and cell death in Arabidopsis and Nicotiana benthamiana[J]. Plant Mol Biol, 2007, 63(2):273-287.
[10] Tan Y F, Toole N O, Taylor N L, et al. Divalent metal ions in plant mitochondria and their role in interactions with proteins and oxidative stress-induced damage to respiratory function[J]. Plant Physiol, 2010, 152: 747-761.
[11] Nouet C, Motte P, Hanikenne M. Chloroplastic and mitochondrial metal homeostasis[J]. Trends Plant Sci, 2011, 16:395-404.
[12] Qin P W, Duan M G, Sun W T, et al. Cloning and prokaryotic expression of a metallothionein cDNA from Chimonanthus praecox (L.)Link[J]. Journal of Beijing Forestry University, 2010, 32: 177-181 (in Chinese). 秦平伟,段明革,孙文婷,等.蜡梅金属硫蛋白基因的克隆与原核表达[J].北京林业大学学报, 2010, 32: 177-181.
/
| 〈 |
|
〉 |