Involvement of AtACO3 in Zn homeostasis

doi:10.7523/j.issn.1002-1175.2013.03.008

Abstract

Abstract:

A full-length cDNA, designated as AtACO3, was isolated from Arabidopsis thaliana. Semi-quantitative RT-PCR revealed that AtACO3 was significantly activated under treatment with 200 μmol/L ZnCl₂ for 1 hour while inhibited under treatment with 200 μmol/L CuCl₂. To investigate the function of the N-terminus of AtACO3, the prokaryotic expression vector pET30a with a fragment of AtACO3 including 1632 bp from the initiation codon was constructed and successfully transformed E. coli. SDS-PAGE indicated that the recombinant N-terminus of AtACO3 was soluble and stable when expressed in E. coli. Additionally, the tolerance of E. coli against Zn²⁺ was found to be enhanced by expressing the N-terminus fragment of AtACO3.

Key words: Zn, Arabidopsis thaliana, aconitase

CLC Number:

X173

LI Yan, WANG Jian-Wu, TAN Jin-Juan, FENG Shan-Shan, CHAI Tuan-Yao. Involvement of AtACO3 in Zn homeostasis[J]. Journal of University of Chinese Academy of Sciences, 2013, 30(3): 329-333.

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