Journal of University of Chinese Academy of Sciences >
The Experimental Study of Electroporation of Cell Membrane Induced by Pulsed Electric Field of High Intensity
Received date: 2004-03-18
Revised date: 2004-09-15
Online published: 2005-07-15
In this paper, cell membrane electroporation induced by pulsed electric field is studied by using liver cancer cells as target cells, Rhodamine Bisothiocyanate-Dextran as fluorescent probes and fluorescence spectrophotometer as measure equipment. The experiments have shown that when the pulse intensity or pulse width increases, the fluorescent intensity will increase accordingly, so will the rate of electroporation of cell membranes. Also we find that electroporation of cell membrane presents memory effects.
XIAO Hua-Juan , YAN Ping , MU Qun . The Experimental Study of Electroporation of Cell Membrane Induced by Pulsed Electric Field of High Intensity[J]. Journal of University of Chinese Academy of Sciences, 2005 , 22(4) : 462 -466 . DOI: 10.7523/j.issn.2095-6134.2005.4.010
[1] James C Weaver. Electroporation in cells and tissues. IEEE Trans on Plasma Science, 2000,28 (1) :24～33
[2] Wang HM, Wang Z. Mechanism and application of cell electroporation and electrofusion. Prog. Biochem. Biophys. 1994,21 (4) :322～326 (in Chinese)
[3] Weaver J C. Electroporationin cells and tissues :a biophysical phenonmenon due to electromagmetic fields. Radio Science,1995,30 (1) :205
[4] Wang BY, Yang JB. Experiment study and mechanism analysis of the bioeffects of electromagnetic pulses. China Science (Volume C),1997,27(1) :35～39 (in Chinese)
[5] Tadej Kotnik, Damijan Miklavcic. Analytical description of transmembrane voltage induced by electric fields on spheroidal cells. Biophysical Journal,2000,79 :670～679
[6] Yao XL, Chen JL. The non2thermal effects of pulsed current electromagnetic fields on bio2membrane. Advanced Technology Electrical Engineering and Energy,2003,22 (2) :76～79 (in Chinese with English abstract)
[7] Liu CJ, Wang BY. Mechanismof specific effects of transient electromagnetic pulses on cell model. J. Biomed. Eng. 1998,15 (3) :294～297 (in Chinese with English abstract)
/
| 〈 |
|
〉 |