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›› 2005, Vol. 22 ›› Issue (4): 462-466.DOI: 10.7523/j.issn.2095-6134.2005.4.010

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The Experimental Study of Electroporation of Cell Membrane Induced by Pulsed Electric Field of High Intensity

XIAO Hua-Juan1,2, YAN Ping1, MU Qun1   

  1. 1. Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing 100080, China;
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
  • Received:2004-03-18 Revised:2004-09-15 Online:2005-07-15

Abstract:

In this paper, cell membrane electroporation induced by pulsed electric field is studied by using liver cancer cells as target cells, Rhodamine Bisothiocyanate-Dextran as fluorescent probes and fluorescence spectrophotometer as measure equipment. The experiments have shown that when the pulse intensity or pulse width increases, the fluorescent intensity will increase accordingly, so will the rate of electroporation of cell membranes. Also we find that electroporation of cell membrane presents memory effects.

Key words: electroporation of cell membrane, high intense pulsed electric field, biological effect

CLC Number: