Effects of nitrogen and phosphorus application on soil microbial biomass carbon and nitrogen contents on an alpine grassland on Tibetan Plateau

ZHAO Guoqiang; WANG Shuping; CUI Xiaoyong; DONG Junfu; CHANG Xuhui; WANG Shiping

doi:10.7523/j.issn.2095-6134.2018.03.017

Journal of University of Chinese Academy of Sciences >

2018 , Vol. 35 >Issue 3: 417 - 424

DOI: https://doi.org/10.7523/j.issn.2095-6134.2018.03.017

Effects of nitrogen and phosphorus application on soil microbial biomass carbon and nitrogen contents on an alpine grassland on Tibetan Plateau

ZHAO Guoqiang ,
WANG Shuping ,
CUI Xiaoyong ,
DONG Junfu ,
CHANG Xuhui ,
WANG Shiping

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1 College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China;
2 College of Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;
3 Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China

Received date: 2017-03-16

Revised date: 2017-05-03

Online published: 2018-05-15

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Abstract

Soil microbes play an important role in maintaining and improving plant productivity as well as soil quality. Based on a two-year field experiment of fertilizer application to an alpine steppe on the Tibetan Plateau, effects of nitrogen and phosphorus addition on soil microbial biomass carbon (MBC) and nitrogen (MBN) contents in the upper 30cm soil were studied. The results are given as follows. 1) Nitrogen and phosphate addition influenced soil MBC content mainly in the 0-10cm soil layer, and the content was 1.67 times and 1.28 times the contents in the 10-20cm and 20-30cm soil layers, respectively. 2) Sole nitrogen addition significantly decreased soil MBC and MBN contents, whereas nitrogen application in addition to phosphorus fertilization increased soil MBC and MBN contents. 3) Phosphorus fertilization alone had no significant effect on soil MBC and MBN contents. Combined with nitrogen fertilizer at the N₂ level of 15g N/m², phosphorus application at the levels of 15 and 30g P₂O₅/m² (i.e., P₂ and P₃ levels) significantly increased MBC content in the 0-10cm soil layer and MBN content in the 10-20cm soil layer, as compared with control treatment. Furthermore, MBN content in the 10-20cm soil layer was also significantly higher in the N₂P₁ (15g N/m² and 7.5g P₂O₅/m²) treatment than CK treatment.

Key words： alpine steppe; phosphorus addition; microbial biomass carbon; microbial biomass nitrogen

Cite this article

ZHAO Guoqiang , WANG Shuping , CUI Xiaoyong , DONG Junfu , CHANG Xuhui , WANG Shiping . Effects of nitrogen and phosphorus application on soil microbial biomass carbon and nitrogen contents on an alpine grassland on Tibetan Plateau[J]. Journal of University of Chinese Academy of Sciences, 2018 , 35(3) : 417 -424 . DOI: 10.7523/j.issn.2095-6134.2018.03.017

References

[1] 周丽霞, 丁明懋. 土壤微生物学特性对土壤健康的指示作用[J]. 生物多样性, 2007, 15(2):162-171.
[2] 白清云. 土壤微生物群落结构的化学估价方法[J]. 农业环境保护, 1997, 16(6):13-17,26.
[3] Gregorich E G, Liang B C, Drury C F, et al. Elucidation of the source and turnover of water soluble and microbial biomass carbon in agricultural soils[J]. Soil Biology & Biochemistry, 2000, 32(5):81-87.
[4] Anderson T H, Domsch K H. Ratios of microbial biomass carbon to total organic carbon in arable soils[J]. Soil Biology and Biochemistry, 1989, 21(4):471-479.
[5] Powlson D S, Prookes P C, Christensen B T. Measurement of soil microbial biomass provides an early indication of changes in total soil organic matter due to straw incorporation[J]. Soil Biology and Biochemistry, 1987, 19(2):159-164.
[6] Sparling G P. Ratio of microbial biomass carbon to soil organic carbon as a sensitive indicator of changes in soil organic matter[J]. Soil Research, 1992, 30(2):195-207.
[7] 毛绍娟, 吴启华, 祝景彬, 等. 藏北高寒草原群落维持性能对封育年限的响应[J]. 草业学报, 2015, 24(1):21-30.
[8] 孙鸿烈, 郑度, 姚檀栋, 等. 青藏高原国家生态安全屏障保护与建设[J]. 地理学报, 2012, 67(1):3-12.
[9] Kautz T, Wirth S, Ellmer F. Microbial activity in a sandy arable soil is governed by the fertilization reuirne[J]. European Journal of Soil Biology, 2004, 40(2):87-94.
[10] Marinari S, Masciandaro G, Ceccanti B, et al. Influence of organic and mineral fertilisers on soil biological and physical properties[J]. Bioresource Technology, 2000, 72(1):9-17.
[11] Jiang X, Shi X, Wright A L. Seasonal variability of microbial biomass associated with aggregates in a rice-based ecosystem[J]. European Journal of Soil Biology, 2013, 48(56):84-88.
[12] Liu M, Hu F, Chen X, et al. Organic amendments with reduced chemical fertilizer promote soil microbial development and nutrient availability in a subtropical paddy field:The influence of quantity, type and application time of organic amendments[J]. Applied Soil Ecology, 2009, 42(2):166-175.
[13] Mandal A, Patra A K, Singh D, et al. Effect of long-term application of manure and fertilizer on biological and biochemical activities in soil during crop development stages[J]. Bioresource Technology, 2007, 98(18):3585-3592.
[14] Tamilselvi S M, Chinnadurai C, Ilamurugu K, et al. Effect of long-term nutrient managements on biological and biochemical properties of semi-arid tropical Alfisol during maize crop development stages[J]. Ecological Indicators, 2015,48(8):76-87.
[15] 徐永刚, 宇万太, 马强, 等. 长期不同施肥制度对潮棕壤微生物生物量碳、氮及细菌群落结构的影响[J]. 应用生态学报, 2010, 21(8):2078-2085.
[16] 王文锋, 李春花, 黄绍文, 等. 不同施肥模式对设施菜田土壤微生物量碳、氮的影响[J]. 植物营养与肥料学报, 2016, 22(05):1286-1297.
[17] Gallardo A, Schlesinger W H. Factors limiting microbial biomass in the mineral soil and forest floor of a warm-temperate forest[J]. Soil Biology and Biochemistry, 1994, 26(10):1409-1415.
[18] Frey S D, Knorr M, Parrent J L, et al. Chronic nitrogen enrichment affects the structure and function of the soil microbial community in temperate hardwood and pine forests[J]. Forest Ecology and Management, 2004, 196(1):159-171.
[19] Compton J E, Watrud L S, Porteous L A, et al. Response of soil microbial biomass and community composition to chronic nitrogen additions at Harvard forest[J]. Forest Ecology and Management, 2004, 196(1):143-158.
[20] Bonde T A, Schnürer J, Rosswall T. Microbial biomass as a fraction of potentially mineralizable nitrogen in soils from long-term field experiments[J]. Soil Biology and Biochemistry, 1988, 20(4):447-452.
[21] Ross D J. Influence of soil mineral nitrogen content on soil respiratory activity and measurements of microbial carbon and nitrogen by fumigation-incubation procedures[J]. Soil Research, 1990, 28(2):311-321.
[22] Biederbeck V O, Campbell C A, Zentner R P. Effect of crop rotation and fertilization on some biological properties of a loam in southwestern Saskatchewan[J]. Canadian Journal of Soil Science, 1984, 64(3):355-367.
[23] 陈丹梅, 刘益仁, 段玉琪,等.长期施肥对作物产量、土壤养分及真菌群落的影响[C]//第八次全国土壤生物与生物化学学术研讨会暨第三次全国土壤健康学术研讨会, 广东河源, 2015:5-6.
[24] 付宇佳.施肥对泡桐人工林土壤微生物特性及酶活性的影响[D].长沙:中南林业科技大学, 2015.
[25] 耿赛男.豆科绿肥对旱坡地紫色土地力提升的机理研究[D].重庆:西南大学, 2015.
[26] 赵晓琛, 皇甫超河, 刘红梅, 等. 贝加尔针茅草原土壤酶活性及微生物量碳氮对养分添加的响应[J]. 草地学报, 2016, 24(1):47-53.
[27] 丁玲玲, 祁彪, 尚占环,等. 东祁连山不同高寒草地型土壤微生物数量分布特征研究[J]. 农业环境科学学报, 2007, 26(6):2104-2111.
[28] 卢虎, 姚拓, 李建宏,等. 高寒地区不同退化草地植被和土壤微生物特性及其相关性研究[J]. 草业学报, 2015, 24(5):34-43.
[29] 贺凤鹏, 曾文静, 王曌迪, 等. 温带草原退化对土壤剖面微生物学特征的影响[J]. 微生物学通报, 2016, 43(3):702-711.
[30] Dentener F, Drevet J, Lamarque J F, et al. Nitrogen and sulfur deposition on regional and global scales:a multimodel evaluation[J]. Global Biogeochemical Cycles, 2006, 20(4):1-21.
[31] Reich P B. Elevated CO₂ reduces losses of plant diversity caused by nitrogen deposition[J]. Science, 2009, 326(5958):1399-1402.
[32] Vourlitis G L, Pasquini S C. Experimental dry-season N deposition alters species composition in southern Californian mediterranean-type shrublands[J]. Ecology, 2009, 90(8):2183-2189.
[33] 徐瑶, 何政伟, 陈涛. 西藏班戈县草地退化动态变化及其驱动力分析[J]. 草地学报, 2011, 19(3):377-380.
[34] 黄昌勇. 土壤学[M]. 北京:中国农业出版社,2000.
[35] 蔡琼, 丁贵杰. 黔中地区连栽马尾松林对土壤微生物的影响[J]. 南京林业大学学报(自然科学版), 2006, 30(3):131-133.
[36] 裴雪霞, 周卫, 梁国庆,等. 长期施肥对黄棕壤性水稻土生物学特性的影响[J]. 中国农业科学, 2010, 43(20):4198-4206.
[37] Jenkinson D S, Davidson S A, Powlson D S. Adenosine-triphosphate and microbial biomass in soil[J]. Soil Biology & Biochemistry, 1979, 11(5):521-527.
[38] Bossio D A, Scow K M, Gunapala N, et al. Determinants of soil microbial communities:effects of agricultural management, season, and soil type on phospholipid fatty acid profiles[J]. Microbial Ecology, 1998, 36(1):1-12.
[39] 白震, 张明, 闫颖,等. 长期施肥对农田黑土微生物活力与群落结构的影响[J]. 土壤学报, 2009, 46(1):107-116.
[40] Hart S C, Stark J M. Nitrogen limitation of the microbial biomass in an old-growth forest soil[J]. Ecoscience, 1997, 4(1):91-98.
[41] Insam H, Mitchell C C, Dormaar J F. Relationship of soil microbial biomass and activity with fertilization practice and crop yield of three ultisols[J]. Soil Biology and Biochemistry, 1991, 23(5):459-464.
[42] Goyal S, Chander K, Mundra M C, et al. Influence of inorganic fertilizers and organic amendments on soil organic matter and soil microbial properties under tropical conditions[J]. Biology and Fertility of Soils, 1999, 29(2):196-200.
[43] Simek M, Hopkins D W, Kalcik J, et al. Biological and chemical properties of arable soils affected by long-term organic and inorganic fertilizer applications[J]. Biology and Fertility of Soils, 1999, 29(3):300-308.
[44] Puri G, Ashman M R. Relationship between soil microbial biomass and gross N mineralisation[J]. Soil Biology & Biochemistry, 1998, 30(2):251-256.
[45] Schnürer J, Rosswall T. Mineralization of nitrogen from 15 N labelled fungi, soil microbial biomass and roots and its uptake by barley plants[J]. Plant and Soil, 1987, 102(1):71-78.
[46] 胡昱欣, 李育松, 卞建民, 等. 一定灌施条件下大安灌区土壤氮素迁移规律[J]. 吉林农业大学学报, 2013,35(3):324-327,345.
[47] 刘金山, 戴健, 刘洋,等. 过量施氮对旱地土壤碳、氮及供氮能力的影响[J]. 植物营养与肥料学报, 2015, 21(1):112-120.

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