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Photoregulating primer extension using azobenzene linked DNA template

  • JI Heming ,
  • KONG Dejia ,
  • MO Mengwu ,
  • LEI Huajun ,
  • CHEN Lu ,
  • ZHAO Ruiqi ,
  • WANG Wei ,
  • HE Yujian ,
  • FENG Lutian ,
  • WU Li
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  • 1. College of Applied Chemistry, Shenyang University of Chemical Technology, Shenyang 11014;
    2. College of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;
    3. College of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang 330013, China

Received date: 2017-11-17

  Revised date: 2018-03-06

  Online published: 2019-01-15

Abstract

It has been a hot topic in recent years to carry out a series of life activities by artificial reversible manipulation of specific gene expression. In this work, the photocontrol of primer extension directed by azobenzene modified DNA templates was investigated. DNA templates attached by protective ODNs with 5, 6, 7, and 8 complementary bases through 4,4'-bis(hydroxymethyl) azobenzene were systematically evaluated in photoregulation of the primer extension without or with UV irradiation. The results showed high efficiencies of C3 with 7 protected bases and C2 with 6-base short chains for photoregulating Pri.15 and Pri.17, respectively. Especially, for C3, when catalyzed by Vent DNA polymerase, primer extension efficiency had one-fold increase upon UV irradiation. Similarly, for C2, the extension efficiency reached 91.4% with UV irradiation and achived 84% increase, compared to that without UV irradiation, although the background of primer extension in the dark was a little high. This work provides a new strategy or means for studying the gene function and gene expression network and for exploring the occurrence and development of disease at the molecular level.

Cite this article

JI Heming , KONG Dejia , MO Mengwu , LEI Huajun , CHEN Lu , ZHAO Ruiqi , WANG Wei , HE Yujian , FENG Lutian , WU Li . Photoregulating primer extension using azobenzene linked DNA template[J]. Journal of University of Chinese Academy of Sciences, 2019 , 36(1) : 38 -47 . DOI: 10.7523/j.issn.2095-6134.2019.01.007

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