Journal of University of Chinese Academy of Sciences >
Distribution and evolution of certain antibiotic resistance genes in different environmental media in Hulunbuir Grassland
Received date: 2024-11-13
Revised date: 2025-03-04
Online published: 2025-04-09
Supported by
the National Natural Science Foundation of China(41775135);the National Natural Science Foundation of China(42275138)
We measured the relative abundance of antibiotic resistance genes （ARGs） related to the treatment of brucellosis by quantitative real-time polymerase chain reaction （PCR）， characterized the bacterial composition by 16S rRNA gene sequencing， and analysed the relationships between ARGs and bacteria by network analyses in multiple environmental media samples （rainwater， topsoil and PM2.5） collected in the summers from 2019 to 2021. The three environmental media of Hulunbuir Grassland were contaminated with some degree of ARGs with the highest ARG abundance in the topsoil. Significant relationships were observed between ARGs and bacteria， thereby promoting the spread of antibiotic resistance. Extensive distribution of ARG carrying bacteria in atmospheric particles， rainwater， and soil may also increase potential ARG exposure risks. These findings are valuable in understanding the ARG pollution levels and distribution characteristics in three environmental media， which can aid to design and implement policies to curb ARGs in local areas.
Key words： ARGs; distribution; abundance; evolution; multiple environmental media; Hulunbuir Grassland
Yuanyuan LI , Li GUO , Rui DU , Hua ZHAO , Zeyu JIA . Distribution and evolution of certain antibiotic resistance genes in different environmental media in Hulunbuir Grassland[J]. Journal of University of Chinese Academy of Sciences, 2026 , 43(3) : 336 -349 . DOI: 10.7523/j.ucas.2025.007
| [1] | Yang Y Y， Liu G H， Ye C， et al. Bacterial community and climate change implication affected the diversity and abundance of antibiotic resistance genes in wetlands on the Qinghai-Tibetan Plateau［J］. Journal of Hazardous Materials， 2019， 361： 283-293. DOI：10.1016/j.jhazmat. 2018.09.002 . |
| [2] | Song M K， Song D D， Jiang L F， et al. Large-scale biogeographical patterns of antibiotic resistome in the forest soils across China［J］. Journal of Hazardous Materials， 2021， 403： 123990. DOI：10. 1016/j.jhazmat.2020.123990 . |
| [3] | Abramova A， Berendonk T U， Bengtsson-Palme J. A global baseline for qPCR-determined antimicrobial resistance gene prevalence across environments［J］. Environment International， 2023， 178： 108084. DOI：10.1016/j.envint.2023.108084 . |
| [4] | Zhou R J， Zeng S Z， Hou D W， et al. Occurrence of human pathogenic bacteria carrying antibiotic resistance genes revealed by metagenomic approach： a case study from an aquatic environment［J］. Journal of Environmental Sciences， 2019， 80： 248-256. DOI：10.1016/j.jes.2019.01.001 . |
| [5] | Zhu G B， Wang X M， Yang T， et al. Air pollution could drive global dissemination of antibiotic resistance genes［J］. The ISME Journal， 2021， 15（1）： 270-281. DOI：10.1038/s41396-020-00780-2 . |
| [6] | Aparicio-Blanco J， Vishwakarma N， Lehr C M， et al. Antibiotic resistance and tolerance： what can drug delivery do against this global threat？［J］. Drug Delivery and Translational Research， 2024， 14（6）： 1725-1734. DOI：10.1007/s13346-023-01513-6 . |
| [7] | Wang Q， Guo S Y， Hou Z L， et al. Rainfall facilitates the transmission and proliferation of antibiotic resistance genes from ambient air to soil［J］. Science of The Total Environment， 2021， 799： 149260. DOI：10.1016/j.scitotenv.2021. 149260 . |
| [8] | Mariappan V， Vellasamy K M， Mohamad N A， et al. OneHealth approaches contribute towards antimicrobial resistance： Malaysian perspective［J］. Frontiers in Microbiology， 2021， 12： 718774. DOI：10.3389/fmicb.2021.718774 . |
| [9] | Ben Y J， Fu C X， Hu M， et al. Human health risk assessment of antibiotic resistance associated with antibiotic residues in the environment： a review［J］. Environmental Research， 2019， 169： 483-493. DOI：10.1016/j.envres.2018.11.040 . |
| [10] | Dafale N A， Srivastava S， Purohit H J. Zoonosis： an emerging link to antibiotic resistance under "one health approach"［J］. Indian Journal of Microbiology， 2020， 60（2）： 139-152. DOI：10. 1007/s12088-020-00860-z . |
| [11] | Zhu Y G， Zhao Y， Li B， et al. Continental-scale pollution of estuaries with antibiotic resistance genes［J］. Nature Microbiology， 2017， 2： 16270. DOI：10.1038/nmicrobiol.2016.270 . |
| [12] | Li J， Cao J J， Zhu Y G， et al. Global survey of antibiotic resistance genes in air［J］. Environmental Science & Technology， 2018， 52（19）： 10975-10984. DOI：10.1021/acs.est.8b02204 . |
| [13] | Xie J W， Jin L， Wu D， et al. Inhalable antibiotic resistome from wastewater treatment plants to urban areas： bacterial hosts， dissemination risks， and source contributions［J］. Environmental Science & Technology， 2022， 56（11）： 7040-7051. DOI：10. 1021/acs.est.1c07023 . |
| [14] | Zhu D， Chen Q L， Ding J， et al. Antibiotic resistance genes in the soil ecosystem and planetary health： Progress and prospect ［J］. Scientia Sinica Vitae， 2019， 49（12）： 1652-1663. DOI：10.1360/SSV-2019-0267. （in Chinese） |
| [15] | Liao Z H， Ren M Z， Sun J R， et al. Health risk assessment of Pb emissions from MSW incineration plants ［J］. Journal of University of Chinese Academy of Sciences， 2014， 31（3）： 410-417. DOI：10.7523/j.issn.2095-6134. 2014.03.017. （in Chinese） |
| [16] | Song L， Jiang G Y， Wang C， et al. Effects of antibiotics consumption on the behavior of airborne antibiotic resistance genes in chicken farms［J］. Journal of Hazardous Materials， 2022， 437： 129288. DOI：10.1016/j.jhazmat.2022.129288 . |
| [17] | Di Cesare A， Eckert E M， Rogora M， et al. Rainfall increases the abundance of antibiotic resistance genes within a riverine microbial community［J］. Environmental Pollution， 2017， 226： 473-478. DOI：10.1016/j.envpol.2017.04.036 . |
| [18] | Zhang Y， Xu Z X， Chu W H， et al. Tracking the source of antibiotic resistome in the stormwater network drainage in the presence of sewage illicit connections［J］. Science of The Total Environment， 2024， 912： 168989. DOI：10.1016/j.scitotenv. 2023. 168989 . |
| [19] | Huijbers P M C， Blaak H， de Jong M C M， et al. Role of the environment in the transmission of antimicrobial resistance to humans： a review［J］. Environmental Science & Technology， 2015， 49（20）： 11993-12004. DOI：10.1021/acs.est. 5b02566 . |
| [20] | Zhao X， Wang J H， Zhu L S， et al. Field-based evidence for enrichment of antibiotic resistance genes and mobile genetic elements in manure-amended vegetable soils［J］. Science of The Total Environment， 2019， 654： 906-913. DOI：10. 1016/j.scitotenv.2018.10.446 . |
| [21] | Huang F Y， Zhou S Y D， Wang J N， et al. Profiling of antibiotic resistance genes in different croplands ［J］. Environmental Science， 2021， 42（6）： 2975-2980. DOI：10.13227/j.hjkx.202009091. （in Chinese） |
| [22] | Zhang T， Zhang M， Zhang X X， et al. Tetracycline resistance genes and tetracycline resistant lactose-fermenting Enterobacteriaceae in activated sludge of sewage treatment plants［J］. Environmental Science & Technology， 2009， 43（10）： 3455-3460. DOI：10.1021/es803309m . |
| [23] | Gao M， Jia R Z， Qiu T L， et al. Size-related bacterial diversity and tetracycline resistance gene abundance in the air of concentrated poultry feeding operations［J］. Environmental Pollution， 2017， 220： 1342-1348. DOI：10.1016/j.envpol.2016.10.101 . |
| [24] | Chen H Y， Li Y Z， Sun W C， et al. Characterization and source identification of antibiotic resistance genes in the sediments of an interconnected river-lake system［J］. Environment International， 2020， 137： 105538. DOI：10.1016/j.envint.2020.105538 . |
| [25] | Gudda F O， Waigi M G， Odinga E S， et al. Antibiotic-contaminated wastewater irrigated vegetables pose resistance selection risks to the gut microbiome［J］. Environmental Pollution， 2020， 264： 114752. DOI：10.1016/j.envpol.2020.114752 . |
| [26] | Zhao Y， Yang Q E， Zhou X， et al. Antibiotic resistome in the livestock and aquaculture industries： status and solutions［J］. Critical Reviews in Environmental Science and Technology， 2021， 51（19）： 2159-2196. DOI：10.1080/10643389.2020. 1777815 . |
| [27] | Zhang S J， Du R， Chen H L， et al. Characteristics and distribution of efficient ice nucleating particles in rainwater and soil［J］. Atmospheric Research， 2020， 246： 105129. DOI：10.1016/j.atmosres.2020. 105129 . |
| [28] | Kozich J J， Westcott S L， Baxter N T， et al. Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform［J］. Applied and Environmental Microbiology， 2013， 79（17）： 5112-5120. DOI：10. 1128/AEM.01043-13 . |
| [29] | Du P R， Du R， Lu Z D， et al. Variation of bacterial and fungal community structures in PM2.5 collected during the 2014 APEC summit periods［J］. Aerosol and Air Quality Research， 2018， 18（2）： 444-455. DOI：10.4209/aaqr.2017.07.0238 . |
| [30] | Anitha P， Anbarasu A， Ramaiah S. Computational gene network study on antibiotic resistance genes of Acinetobacter baumannii ［J］. Computers in Biology and Medicine， 2014， 48： 17-27. DOI：10.1016/j.compbiomed.2014.02.009 . |
| [31] | Muurinen J， Stedtfeld R， Karkman A， et al. Influence of manure application on the environmental resistome under Finnish agricultural practice with restricted antibiotic use［J］. Environmental Science & Technology， 2017， 51（11）： 5989-5999. DOI：10.1021/acs.est.7b00551 . |
| [32] | Merino L， Darnerud P O， Toldrá F， et al. Time-dependent depletion of nitrite in pork/beef and chicken meat products and its effect on nitrite intake estimation［J］. Food Additives & Contaminants： Part A， 2016， 33（2）： 186-192. DOI：10.1080/19440049.2015.1125530 . |
| [33] | Rossi R， Saluti G， Moretti S， et al. Multiclass methods for the analysis of antibiotic residues in milk by liquid chromatography coupled to mass spectrometry： a review［J］. Food Additives & Contaminants： Part A， 2018， 35（2）： 241-257. DOI：10.1080/19440049.2017. 1393107 . |
| [34] | Yang S F， Lin C F， Lin A Y C， et al. Sorption and biodegradation of sulfonamide antibiotics by activated sludge： experimental assessment using batch data obtained under aerobic conditions［J］. Water Research， 2011， 45（11）： 3389-3397. DOI：10.1016/j.watres.2011.03.052 . |
| [35] | Carvalho I T， Santos L. Antibiotics in the aquatic environments： a review of the European scenario［J］. Environment international， 2016， 94： 736-757. DOI：10.1016/j.envint.2016.06.025 . |
| [36] | Zhang K， Xin R， Zhao Z， et al. Mobile genetic elements are the major driver of high antibiotic resistance genes abundance in the upper reaches of Huaihe River Basin［J］. Journal of Hazardous Materials， 2021， 401： 123271. DOI：10.1016/j. jhazmat.2020.123271 . |
| [37] | Makowska N， Koczura R， Mokracka J. Class 1 integrase， sulfonamide and tetracycline resistance genes in wastewater treatment plant and surface water［J］. Chemosphere， 2016， 144： 1665-1673. DOI：10.1016/j.chemosphere.2015.10.044 . |
| [38] | Niu Z G， Zhang K， Zhang Y. Occurrence and distribution of antibiotic resistance genes in the coastal area of the Bohai Bay， China［J］. Marine Pollution Bulletin， 2016， 107（1）： 245-250. DOI：10.1016/j.marpolbul.2016.03.064 . |
| [39] | Marti R， Scott A， Tien Y C， et al. Impact of manure fertilization on the abundance of antibiotic-resistant bacteria and frequency of detection of antibiotic resistance genes in soil and on vegetables at harvest［J］. Applied and Environmental Microbiology， 2013， 79（18）： 5701-5709. DOI：10. 1128/AEM.01682-13 . |
| [40] | Tian H Y， Liu J W， Sun J B， et al. Cross-media migration behavior of antibiotic resistance genes （ARGs） from municipal wastewater treatment systems （MWTSs）： fugitive characteristics， sharing mechanisms， and aerosolization behavior［J］. Science of The Total Environment， 2023， 893： 164710. DOI：10.1016/j.scitotenv. 2023. 164710 . |
| [41] | Wang Y Z， Wang C， Song L. Distribution of antibiotic resistance genes and bacteria from six atmospheric environments： exposure risk to human［J］. Science of The Total Environment， 2019， 694： 133750. DOI：10.1016/j.scitotenv. 2019. 133750 . |
| [42] | Xie J W， Jin L， Luo X S， et al. Seasonal disparities in airborne bacteria and associated antibiotic resistance genes in PM2.5 between urban and rural sites［J］. Environmental Science & Technology Letters， 2018， 5（2）： 74-79. DOI：10.1021/acs.estlett. 7b00561 . |
| [43] | Cáliz J， Subirats J， Triadó-Margarit X， et al. Global dispersal and potential sources of antibiotic resistance genes in atmospheric remote depositions［J］. Environment International， 2022， 160： 107077. DOI：10.1016/j.envint.2022.107077 . |
| [44] | Maddamsetti R， Yao Y， Wang T， et al. Duplicated antibiotic resistance genes reveal ongoing selection and horizontal gene transfer in bacteria［J］. Nature Communications， 2024， 15（1）： 1449. DOI：10.1038/s41467-024-45638-9 . |
| [45] | Xie J W， Jin L， He T T， et al. Bacteria and antibiotic resistance genes （ARGs） in PM2.5 from China： implications for human exposure［J］. Environmental Science & Technology， 2019， 53（2）： 963-972. DOI：10.1021/acs.est.8b04630 . |
| [46] | Bai H， He L Y， Wu D L， et al. Spread of airborne antibiotic resistance from animal farms to the environment： dispersal pattern and exposure risk［J］. Environment International， 2022， 158： 106927. DOI：10.1016/j.envint.2021.106927 . |
| [47] | Dey H， Vasudevan K， Dasegowda K R， et al. An integrated gene network analysis to decode the multi-drug resistance mechanism in Klebsiella pneumoniae ［J］. Microbial Pathogenesis， 2022， 173： 105878. DOI：10.1016/j.micpath.2022.105878 . |
| [48] | Hu Y F， Yang X， Qin J J， et al. Metagenome-wide analysis of antibiotic resistance genes in a large cohort of human gut microbiota［J］. Nature Communications， 2013， 4： 2151. DOI：10.1038/ ncomms3151 . |
| [49] | Hernando-Amado S， Coque T M， Baquero F， et al. Defining and combating antibiotic resistance from One Health and Global Health perspectives［J］. Nature Microbiology， 2019， 4（9）： 1432-1442. DOI：10.1038/s41564-019-0503-9 . |
| [50] | Chen Y F， Ke Y H， Wang Y F， et al. Changes of predominant species/biovars and sequence types of Brucella isolates， Inner Mongolia， China［J］. BMC Infectious Diseases， 2013， 13： 514. DOI：10. 1186/1471-2334-13-514 . |
| [51] | Kong M， Zhang Y， Ma Y， et al. Antibiotics and antibiotic resistance change bacterial community compositions in marine sediments［J］. Environmental Research， 2024， 244： 118005. DOI：10.1016/j.envres.2023.118005 . |
/
| 〈 |
|
〉 |