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中国科学院大学学报 ›› 2012, Vol. 29 ›› Issue (4): 449-454.DOI: 10.7523/j.issn.2095-6134.2012.4.003

• 生命科学 • 上一篇    下一篇

商陆金属硫蛋白基因PaMT的表达分析

王校, 赵会君, 刘慧敏, 柴团耀   

  1. 中国科学院研究生院生命科学学院, 北京 100049
  • 收稿日期:2011-04-26 修回日期:2011-05-19 发布日期:2012-07-15
  • 通讯作者: 柴团耀
  • 基金资助:
    国家高技术研究发展计划(863)(2006AA10Z407),国家转基因生物新品种培育科技重大专项(2009ZX08009-130B)资助

Expression analysis of PaMT from Phytolacca Americana

WANG Xiao, ZHAO Hui-Jun, LIU Hui-Min, CHAI Tuan-Yao   

  1. College of Life Science,Graduate University, Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-04-26 Revised:2011-05-19 Published:2012-07-15

摘要: 从商陆抑制性消减杂交文库中获得金属硫蛋白PaMT,基因全长141 bp,编码46个氨基酸,等电点和分子量分别为3.85和4.7 kD,具有12个半胱氨酸残基:具有典型的金属硫蛋白结构(CXC结构). 进化树和蛋白结构分析表明它属于金属硫蛋白家族. PaMT主要在根中表达,且受重金属胁迫上调其基因表达. 蛋白融合表达表明,PaMT可以提高大肠杆菌对重金属离子的耐受力.

关键词: 商陆, 重金属, 金属硫蛋白

Abstract: A full-length cDNA of metallothionein, designated PaMT, was cloned from SSH libraries. PaMT consists of 141 bp that code 46 amino acids. Classic structure (cxc) of metallothionein and the phylogenetic tree analysis show that PaMT belongs to metallothionein family. PaMT is preferentially expressed in root and its expression is strongly up-regulated by heavy metal stress. PaMT-NusA fusion protein expressed in Coli BL21 plays a role in the tolerance to heavy metal toxicity.

Key words: Phytolacca Americana, heavy metal, metallothionein

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