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›› 2005, Vol. 22 ›› Issue (2): 192-196.DOI: 10.7523/j.issn.2095-6134.2005.2.011

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Optimization of Reaction Conditions in the Asymmetric Synthesis of D-Mandelic Acid Methyl Ester Catalyzed by Saccharomyces Cereviriae

SHENG Jun1,2, GUO Yang-Hao2, SHI Xian-Ai1, XIAO Mei-Tian2, MENG Chun1   

  1. 1. College of Biological Science and Technology, Fuzhou University, Fuzhou 350002, China;
    2. Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, Fuzhou 350002, China
  • Received:2004-06-09 Revised:2004-08-16 Online:2005-03-15

Abstract:

In the process of asymmetric synthesis of D-mandelic acid methyl ester catalyzed by Saccharomyces cereviriae, the most favorite pH for conversion is 7.0.Both the environmental temperature and oxygen concentration all have an important effect on conversion of substrate.Using packed bed reactor filled with immobilized cells to produce D-mandelic acidmethyl ester,25 days later the conversion efficiency can be still sustained in 80 %and the ee value is more than 95 %when the charge was 0.2 mmol (d·g)wet cell.

Key words: Saccharomyces cereviriae, asymmetric synthesis, D-mandelic acid methyl ester, oxygen level

CLC Number: