Simultaneous determination of oxysophocarpine, sophoridine, and matrine by HPLC

doi:10.7523/j.issn.2095-6134.2011.3.018

Abstract

Abstract:

An HPLC method for simultaneous determination of oxysophocarpine, sophoridine, and matrine was proposed. VP-ODS column (150 mm × 4.6 mm, 5 μm) was used in the experiment. Column temperature was 40 ℃, mobile phase was 0.01 mol/L phosphate buffer solution (pH 8.5)-methanol (40 ∶60), UV detection wavelength was 215nm, and flow rate was 1.0 mL/min. The results indicated that the three kinds of alkaloid were separated effectively and showed good linearity within the selected concentration range.

Key words: oxysophocarpine, sophoridine, matrine, HPLC

CLC Number:

R917

WU Zhi-Jiao, ZHAO Hong, XUE Ying, HU Xin-Ming, MAO Yi-Qing, TAN Huan-Ran. Simultaneous determination of oxysophocarpine, sophoridine, and matrine by HPLC[J]. , 2011, 28(3): 398-401.

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