Establishment of Mathematic-Physical Models for Target Immobilization in High-Throughput Screening

doi:10.7523/j.issn.2095-6134.2005.2.009

Abstract

Abstract:

Based on the analysis of the basic process involved in high-throughput screening ( HTS), the present article discusses the possible effect of target immobilizat ion on the screening quality. Judged from intermolecular interactions, the actual process in HTS is more complicated than that in ideal situation. With more or less interactions electrostatic, hydrophobic or hydrogen-bonding can be involved in this process. With the induction derived from these interactions, the immobilization mode of target may be in monolayer or a more complicated mult-i layer form. In addit ion, this article presents the mathematic expressions related to the corresponding interaction forms, including target and target, target and microsphere, and bulk phase and surface phase as well. These expressions are expected to show a certain degree of usability in the future work.

Key words: high-throughput screening of drugs, target immobilization, intermolecular interaction, mathematicphysical model

CLC Number:

R965.1

LI Song-Jun, LIU Bai-Ling . Establishment of Mathematic-Physical Models for Target Immobilization in High-Throughput Screening[J]. , 2005, 22(2): 179-186.

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