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›› 2005, Vol. 22 ›› Issue (2): 187-191.DOI: 10.7523/j.issn.2095-6134.2005.2.010

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Studies on the Chemotaxis of a Bacterium Pseudomonas sp. Using Crude Oil as Carbon Source

JING Gui-Cheng1, GUO Shang-Ping2, YU Li1   

  1. 1. Institute of Porous Flow and Fluid Mechanics, Chinese Academy of Sciences, La ngfang 065007, China;
    2. Research Institute of Petroleum Exploration and Development, PetroChina, Beijing 100083, China
  • Received:2004-06-08 Revised:2004-09-17 Online:2005-03-15

Abstract:

A bacterium Pseudomonas sp. using the crude oil as the carbon source was separated from the production water in an oilfield. Its chemotaxis migration ability was really medium, and its chemotaxis mechanism was particularly described in this paper. In visualized experiments, the ordered motion of microorganism to the oil water interface could be watched, and within the scope of the effect ive nutriment concentration grads, the chemotaxis mot ion velocity was not related with the distance from the interface at all and it was much influenced by the temperature. Meanwhile, in experiments using emulational micro-models, it could be observed that bacteria had become enriched at the orifice wall, oil and water three-phrase boundary, and the forming reason and the beneficial effect on MEOR were also discussed.

Key words: microorganism, chemotaxis, oil water interface, enrichment

CLC Number: