Towards Genetic Engineering Cassava(Manihot esculenta Crantz) for Preventing Premature Leaf Shed

Abstract

Abstract:

To prevent premature leaf shed in cassava, a construct containing an ipt gene driven by a senescence specific promoter (SAG12 )w as successfully transferred into cassava (M Col 22 )via Agrobacterium- mediated transformation method.Mature cotyledons f rom somatic embryos,which had been cultivated to be maturative for 15 days, were cut into pieces and used as explants for transformation.3 days after inoculation with Agrobacterium, the explants were t ransferred onto the organogenesis induction medium (MS basal medium supplemented with 1 mg/ L BAP and 0.5 mg/ L IBA )with 30 mg/ L G418 for selection.Resistant organ structures and shoots appeared in about 3 weeks, the cultures were later transferred to shoot elongation medium and the resistant shoots were finally rooted on MS basal medium without hormones.PCR and Southern analysis confirmed the transgenic nature of some resistant plants.

Key words: SAG12-ipt gene, cassava, transformation

CLC Number:

Q944

Li Hongqing, Li Meiru, Liu Hongxian, Liang Chengye. Towards Genetic Engineering Cassava(Manihot esculenta Crantz) for Preventing Premature Leaf Shed[J]. , 2000, 17(2): 74-80.

References

1 Akiyoshi DE, Klee H, Amasino RM, Nest er EW, Gordon MP.T-DNA of Agrobacterium Tumefaciens Encodes an Enzyme of Cyt okinin Biosynthesis.Proc.Natl Acad Sci USA, 1984, 81 :5994 ～ 5998

2 Barry GF, Rogers SG, Fraley RT, Brand L.Identification of a Cloned Cyt okinin Biosynthetic Gene.Proc.Nat l Acad Sci USA, 1984, 81 : 4776 ～ 4780

3 Devlin RM, Witham FH.Plant Physiology.Boston:Willard Grant Press, 1983.411 ～ 447

4 Gan SS, Amasino RM.Inhibition of Leaf Senescence by Aut oregulated Production of Cytokinin.Science, 1995, 270 :1986～ 1988

5 Gan SS, Amasino RM.Making Sense of Senescence.Plant Physiol.1997, 113 :313～ 319

6 Horgan R.Cytokinins In Advanced Plant Physiology (M.B.Wilkins, Ed.), Pitman, London.1984.53～ 75

7 Klee H, Est elle M.Molecular Genet ic Approaches to Plant Hormone Biology.Annu Rev Plant Physiol Plant Mol Biol, 1991, 42 :529 ～ 551

8 Li H -Q, Huang Y-W, Liang C-Y, Guo J -Y.Improvement of Plant Regeneration From Cyclic Somatic Embryos in Cassava.In:Cassava Biot echnology Netw ork:Proceeding of the 2nd Int ernational Scient if ic Meet ing, Bogor, Indonesia, CIAT Working Document, 1995, 150 : 289 -302

9 Li H -Q, Huang Y -W, Liang C -Y, Guo J -Y, Liu H -X, Pot rykus I, Puonti -Kaerlas J.Regeneration of Cassava Plants Via Shoot Organogenesis.Plant Cell Rep.1998, 17 :410 ～ 414

10 Li H-Q, Sautter C, Pot rykus I, Puonti-Kaerlas J.Genetic Transformation of Cassava (Manihot escu lenta Crantz).Nature Biot echnology 1996, 14:736～ 740

11 Lohman KN, Gan S, John MC, Amasino RM.Molecular Analysis of Mature Leaf Senescence in Arabidopsis thal iana.Physiol Plant, 1994, 92:322 ～ 328

12 Medford JI, Horgan R, El-Sawi Z, Klee HJ.Alterations of Endogeneous Cytokinins in Transgenic Plants Using a Chimeric Isopent enyl Transferase Gene.Plant Cell, 1989, 1 :403 ～ 413

13 Mullins KV, Llewellyn DJ, Hartney VJ, St rauss S, Dennis ES.Regeneration and Transformation of Eucalyptus camaldu lensis.Plant Cell Rep, 1997, 16 :787～ 791

14 Potrykus I, Spangenberg G.Gene Transfer to Plants.Springer-Verlag, Berlin, Germany.1995

15 Schopke C, Franche C, Bogusz D, Chavarriaga P, Fauquet CM, Beachy RN.Transformation in Cassava (Manihot esculenta Crantz ), In Biotechnology in Agriculture and Forest ry.Bajaj, Y.P.S.(ed.).Berlin:Speinger-Verlag, 1993.273 ～ 289

16 Smart CM, Scofield SR, Bevan MW, Dyer TA.Delayed Leaf Senescence in Tobacco Plants Transformed with tmr, a Gene for Cyt okinin Production in Agrobacterium.Plant Cell, 1991, 3 :647 ～ 656

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