Study on the zinc tolerance and translocation mechanisms of “Zhongyou”, a zinc-rich wheat variety

doi:10.7523/j.issn.2095-6134.2017.06.004

Abstract

Abstract: The zinc-rich wheat variety "Zhongyou" was used as research material to study responses of physiological and biochemical changes at the seedling stage under Zn stress by using hydroponic cultivation. The effects of different concentrations of Zn treatments on the cell death phenomenon were observed, and the above ground tissue Zn accumulation and its effect on antioxidant system (SOD, POD, and APX) were studied. TaHMA1(Heavy metal ATPase) was obtained by using 5' and 3'race technologies, and the expression patterns of HMA1, HMA2, PCs, and MT under Zn treatment were studied by using quantitative real-time PCR. Our results are given as follows.This variety has the ability to accumulate high-concentration Zn in above ground tissue. Zn leads to the cell death in root tissue and the inhibition of the SOD activities in leaf tissue, but improves the POD and APX activities. TaHMA1, TaHMA2, and PCs are induced by Zn, and they may play important roles in absorbing and accumulation of Zn in above ground tissue.

Key words: Zn rich wheat variety, race technology, heavy metal ATPase, real-time quantitative PCR

CLC Number:

S512.1

ZHAO Huijun, XU Shuya, ZHANG Xiu. Study on the zinc tolerance and translocation mechanisms of “Zhongyou”, a zinc-rich wheat variety[J]. , 2017, 34(6): 675-683.

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