Cytoprotective effect of Nrf2 expression on hyperglycemia-induced oxidative stress in pancreatic NIT-1β-cells

doi:10.7523/j.issn.2095-6134.2015.03.003

Abstract

Abstract:

It is well known that hyperglycemia results in oxidative stress in diabetes mellitus, which causes pancreatic β cell dysfunction. However, this cytoprotective effect of Nrf2 on hyperglycemia-induced oxidative stress in pancreatic β cell is not fully understood. In the study, reactive oxygen species (ROS) and insulin synthesis and secretion as well as nucleus NF-E2-related factor 2 (Nrf2) expression in NIT-1β cells were determined after treatment of 48 h with low glucose (LG, 5.5 mmol/L), high glucose (HG, 27.6 mmol/L), or LG plus H₂O₂, respectively. We found that HG significantly induced ROS generation and decreased insulin synthesis and secretion in NIT-1β cells. However, HG-induced oxidative damage was improved by nucleus NF-E2-related factor 2 (Nrf2) expression. The results suggested that activation of Nrf2 through promotion of nuclear accumulation plays an important role in protection against hyperglycemia-induced oxidative injury.

Key words: hyerglycemia, pancreatic &beta, cell, oxidative stress, Nrf2

CLC Number:

587.1

NING Mengli, DING Wenjun, ZHANG Fang. Cytoprotective effect of Nrf2 expression on hyperglycemia-induced oxidative stress in pancreatic NIT-1β-cells[J]. , 2015, 32(3): 301-308.

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