Relationships among Kengyilia species assessed by RAPD markers

›› 2000, Vol. 38 ›› Issue (6): 515-521.

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Relationships among Kengyilia species assessed by RAPD markers

ZHOU Yong-Hong, ZHENG You-Liang, YANG Jun-Liang, YEN Chi, JIA Ji-Zeng

Received:1900-01-01 Revised:1900-01-01 Online:2000-11-10
Contact: ZHOU Yong-Hong

Abstract

Abstract:

To assess the interspecific relationships in Kengyilia, fourteen species of Kengyilia were

used for RAPD assay. Of the 34 random primers tested, twenty (58.8%) produced polymorphic

products. A total of 112 products amplified from 14 primers were selected for RAPD analysis.

Eighty-three (74.1%) amplified products were found to be polymorphic among 14 species. Data

were used to generate Jaccard's similarity coefficients and to construct a dendrogram using UPGMA

in the NTSYS computer programs. It is concluded from this study that: (1) There was great genetic

diversity among the 14 species of Kengyilia; (2) Distinct RAPD variations existed between the spe-

cies from the Qinghai-Xizang Plateau and those from Xinjiang; (3) The species with similar morpho-

logical characters and the species from the same areas or neighboring geographical regions were clus-

tered together; (4) RAPD results are comparable with those obtained from studies on morphology

and cytology. RAPD assay is a useful additional method for assessing the relationships among the

species in Kengyilia.

Key words: Kengyilia, RAPD assay, Genetic variation, Relationships, Morphology

ZHOU Yong-Hong, ZHENG You-Liang, YANG Jun-Liang, YEN Chi, JIA Ji-Zeng. Relationships among Kengyilia species assessed by RAPD markers[J]. , 2000, 38(6): 515-521.

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