Purification and Characterization of UGPase

doi:10.7523/j.issn.2095-6134.2004.3.010

Abstract

Abstract:

After isolation and purification of four steps, specific activity of UGPase from Apocynaceae cell is 204 9 U/mg. Compared with crude cell culture, specific activity is 97 times, and recovery yield is 21%. UGPase can be used in the synthesis of UDPG, and it has higher stability. The best available pH of UGPase is about 7 2, the best available temperature about 37℃. Mg²⁺ is necessary for UGPase to use. The high concentration of UTP and Glc 6 P can inhibit the activity of UGPase.

Key words: UGPase, purification, characterization, specific activity

CLC Number:

Q2-33

QI Chao. Purification and Characterization of UGPase[J]. , 2004, 21(3): 345-351.

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